hsf1 flag Search Results


hsf1 flag  (Addgene inc)
88
Addgene inc hsf1 flag
Hsf1 Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gcn4 fragment  (Addgene inc)
92
Addgene inc gcn4 fragment
Gcn4 Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag hsf1 plasmid  (Addgene inc)
93
Addgene inc flag hsf1 plasmid
Flag Hsf1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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influenza virus a puerto rico 8 1934  (Addgene inc)
93
Addgene inc influenza virus a puerto rico 8 1934
Influenza Virus A Puerto Rico 8 1934, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saccharomyces cerevisiae tof1δc  (Addgene inc)
93
Addgene inc saccharomyces cerevisiae tof1δc
Enhancement of chromatin replication is limited by FACT and Tof1 truncations. ( A ) Reaction scheme of the in vitro chromatin replication assay. ( B ) Chromatin replication showing loss of enhancement for SΔC + PΔC and SΔN + P as compared to fl FACT, all at 50 nM. All conditions contained 20 nM fl Tof1-Csm3. Asterix indicates end labelling of nicked plasmid DNA. ( C and D ) Chromatin replication performed as in B, with SΔC-PΔC and SΔN-P titrated up to 400 nM, an 8-fold excess over fl FACT. Middle: Lane profiles for the replication reactions in C and D, respectively, in the absence of FACT (magenta), with fl FACT (teal), and with FACT truncations (blue). ( E ) DNA replication showing activity of <t>Tof1ΔC-Csm3</t> comparable to fl Tof1-Csm3 both at 20 nM. DNA was not chromatinized and no FACT was included. ( F ) Chromatin replication performed as in B, with Tof1ΔC-Csm3, fl FACT was titrated up to 400 nM. Middle: Lane profiles for the replication reactions in F, in the absence of FACT (magenta), with fl FACT and fl Tof1-Csm3 (teal), and Tof1ΔC-Csm3 with fl FACT titration (blue). Data were fit to a Gaussian distribution. The vertical line shows the mean of the distribution. Right: Enhancement of replication (%) based on the mean migration distance between replication reactions in the absence of FACT (0% enhancement, magenta) and with fl FACT (100% enhancement, teal) at 50 nM . Error bars indicate mean ± s.d. from three independent experiments. Recovery of replication enhancement is observed with excess concentration of SΔN-P, and fl FACT in reactions with Tof1ΔC-Csm3, but not with SΔC-PΔC. Lines above gels and on the enhancement charts indicate the lanes for direct comparison with the wild-type condition at 50 nM.
Saccharomyces Cerevisiae Tof1δc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a wsn 1933  (Addgene inc)
92
Addgene inc a wsn 1933
Enhancement of chromatin replication is limited by FACT and Tof1 truncations. ( A ) Reaction scheme of the in vitro chromatin replication assay. ( B ) Chromatin replication showing loss of enhancement for SΔC + PΔC and SΔN + P as compared to fl FACT, all at 50 nM. All conditions contained 20 nM fl Tof1-Csm3. Asterix indicates end labelling of nicked plasmid DNA. ( C and D ) Chromatin replication performed as in B, with SΔC-PΔC and SΔN-P titrated up to 400 nM, an 8-fold excess over fl FACT. Middle: Lane profiles for the replication reactions in C and D, respectively, in the absence of FACT (magenta), with fl FACT (teal), and with FACT truncations (blue). ( E ) DNA replication showing activity of <t>Tof1ΔC-Csm3</t> comparable to fl Tof1-Csm3 both at 20 nM. DNA was not chromatinized and no FACT was included. ( F ) Chromatin replication performed as in B, with Tof1ΔC-Csm3, fl FACT was titrated up to 400 nM. Middle: Lane profiles for the replication reactions in F, in the absence of FACT (magenta), with fl FACT and fl Tof1-Csm3 (teal), and Tof1ΔC-Csm3 with fl FACT titration (blue). Data were fit to a Gaussian distribution. The vertical line shows the mean of the distribution. Right: Enhancement of replication (%) based on the mean migration distance between replication reactions in the absence of FACT (0% enhancement, magenta) and with fl FACT (100% enhancement, teal) at 50 nM . Error bars indicate mean ± s.d. from three independent experiments. Recovery of replication enhancement is observed with excess concentration of SΔN-P, and fl FACT in reactions with Tof1ΔC-Csm3, but not with SΔC-PΔC. Lines above gels and on the enhancement charts indicate the lanes for direct comparison with the wild-type condition at 50 nM.
A Wsn 1933, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Enhancement of chromatin replication is limited by FACT and Tof1 truncations. ( A ) Reaction scheme of the in vitro chromatin replication assay. ( B ) Chromatin replication showing loss of enhancement for SΔC + PΔC and SΔN + P as compared to fl FACT, all at 50 nM. All conditions contained 20 nM fl Tof1-Csm3. Asterix indicates end labelling of nicked plasmid DNA. ( C and D ) Chromatin replication performed as in B, with SΔC-PΔC and SΔN-P titrated up to 400 nM, an 8-fold excess over fl FACT. Middle: Lane profiles for the replication reactions in C and D, respectively, in the absence of FACT (magenta), with fl FACT (teal), and with FACT truncations (blue). ( E ) DNA replication showing activity of Tof1ΔC-Csm3 comparable to fl Tof1-Csm3 both at 20 nM. DNA was not chromatinized and no FACT was included. ( F ) Chromatin replication performed as in B, with Tof1ΔC-Csm3, fl FACT was titrated up to 400 nM. Middle: Lane profiles for the replication reactions in F, in the absence of FACT (magenta), with fl FACT and fl Tof1-Csm3 (teal), and Tof1ΔC-Csm3 with fl FACT titration (blue). Data were fit to a Gaussian distribution. The vertical line shows the mean of the distribution. Right: Enhancement of replication (%) based on the mean migration distance between replication reactions in the absence of FACT (0% enhancement, magenta) and with fl FACT (100% enhancement, teal) at 50 nM . Error bars indicate mean ± s.d. from three independent experiments. Recovery of replication enhancement is observed with excess concentration of SΔN-P, and fl FACT in reactions with Tof1ΔC-Csm3, but not with SΔC-PΔC. Lines above gels and on the enhancement charts indicate the lanes for direct comparison with the wild-type condition at 50 nM.

Journal: Nucleic Acids Research

Article Title: The fork protection complex recruits FACT to reorganize nucleosomes during replication

doi: 10.1093/nar/gkac005

Figure Lengend Snippet: Enhancement of chromatin replication is limited by FACT and Tof1 truncations. ( A ) Reaction scheme of the in vitro chromatin replication assay. ( B ) Chromatin replication showing loss of enhancement for SΔC + PΔC and SΔN + P as compared to fl FACT, all at 50 nM. All conditions contained 20 nM fl Tof1-Csm3. Asterix indicates end labelling of nicked plasmid DNA. ( C and D ) Chromatin replication performed as in B, with SΔC-PΔC and SΔN-P titrated up to 400 nM, an 8-fold excess over fl FACT. Middle: Lane profiles for the replication reactions in C and D, respectively, in the absence of FACT (magenta), with fl FACT (teal), and with FACT truncations (blue). ( E ) DNA replication showing activity of Tof1ΔC-Csm3 comparable to fl Tof1-Csm3 both at 20 nM. DNA was not chromatinized and no FACT was included. ( F ) Chromatin replication performed as in B, with Tof1ΔC-Csm3, fl FACT was titrated up to 400 nM. Middle: Lane profiles for the replication reactions in F, in the absence of FACT (magenta), with fl FACT and fl Tof1-Csm3 (teal), and Tof1ΔC-Csm3 with fl FACT titration (blue). Data were fit to a Gaussian distribution. The vertical line shows the mean of the distribution. Right: Enhancement of replication (%) based on the mean migration distance between replication reactions in the absence of FACT (0% enhancement, magenta) and with fl FACT (100% enhancement, teal) at 50 nM . Error bars indicate mean ± s.d. from three independent experiments. Recovery of replication enhancement is observed with excess concentration of SΔN-P, and fl FACT in reactions with Tof1ΔC-Csm3, but not with SΔC-PΔC. Lines above gels and on the enhancement charts indicate the lanes for direct comparison with the wild-type condition at 50 nM.

Article Snippet: Saccharomyces cerevisiae Tof1ΔC (1-937 aa) and Csm3 were amplified from genomic template into 12-Ade-B and 12-Trp-U vectors (a kind gift from S. Gradia, UC Berkeley, Addgene plasmids #48298 and #48303, Addgene) following standard genetic procedures.

Techniques: In Vitro, Plasmid Preparation, Activity Assay, Titration, Migration, Concentration Assay, Comparison